Subsequently, Zhou et al. showed that ischemia increases LRRC8A-dependent VRAC activity in hippocampal neurons, which enhances glutamate release and contributes to ischemic brain injury (Zhou et al., 2020), and that mice with conditional knockout of the LRRC8A gene had significantly lower infarct volume and neurological severity scores than wild-type mice after middle cerebral artery occlusion cerebral infarction (Zhou et al., 2020). This evidence concerns the gene LRRC8A and ischemia.