Our results comparing the different clones show that the scFv derived from the 4H5 clone of anti‐human EphA2 antibody, which is a humanised version of the EA2 clone of the anti‐mouse EphA2 antibody,49 was most effective for the recognition and killing of sarcoma tumour cells by EphA2‐CAR‐NK cells in vitro. Here, EPHA2 is linked to sarcoma.