NEFM and reading: A total of 22 MRDEGs were obtained: SPTBN2, MYLK, CA2, NEFM, CAV1, MYH11, MAPK15, LRCH2, FLNC, PSAT1, EPB41L3, TUBB4A, IGF2BP1, CDKN2A, KIF1A, NEXN, DPYSL5, MIF, IL24, AHSG, IGF2BP3 and ALB. The heatmap results showed that the expression of MRDEGs differed between READ and normal control samples.