Subsequently, NEK8 protein and mRNA expression were evaluated across four GC cell lines (AGS, SGC7901, MGC803, and HGC27) as well as the GES-1 cell line, with results showing a marked increase in NEK8 levels in the GC cell lines relative to GES-1 (Fig. 1B–D). The gene discussed is NEK8; the disease is gastric cancer.