To explore the role of SPAG5 antagonism as a possible treatment strategy in AML, its role in AML pathogenesis must first be established; thus, to search for additional potential therapeutic targets and gain insights into the role of SPAG5 in AML progression, a high-throughput Swarm-based deep neural network methodology was applied to evaluate SPAG5 as a potential therapeutic target as a regulator of the TP53 pathway in AML. This evidence concerns the gene SPAG5 and acute myeloid leukemia.