SMYD3 and head and neck squamous cell carcinoma: To identify direct gene targets transcriptionally regulated by SMYD3 that could provide mechanistic insights into the observed phenotypes of decreased proliferative, colony formation capacity and decreased invasive potential of HPV-negative HNSCC cells after SMYD3 depletion, genome-wide mapping of SMYD3 and its histone enzymatic end-product H3K4me33 was pursued in parental HN-6 and SMYD3 KO cells (5–3 cell line) using CUT&RUN assays.