We examined the expression of pro‐ and antiapoptotic proteins—Bax, Bcl‐2, Bcl‐2l, Bak‐1, apoptosis/necrosis‐related proteins ripk1, ripk3, and p53 and caspases which related to apoptosis—3, 8, 9, and parp1 in neuron‐glial culture, fibroblasts and BT‐474 cancer cells by RT‐qPCR analysis. The gene discussed is PARP1; the disease is cancer.