Thus, even though additional binding of Galectin-3 to the cell surface of neutrophils is possible (neutrophils reach saturation at around 8 μM, as compared to a maximum of 4.4 nM measured in HGSC ascites in this study and 0.5 nM in healthy donor plasma), it will not induce more ROS production when high amounts of Galectin-3C is bound to the cell surface (40). Here, LGALS3 is linked to Ascites.