To better illustrate the effect of RTN4IP1 on ESCC development, we generated esophageal‐specific Rtn4ip1‐knockout (CKO) mice by crossing Rtn4ip1fl/fl mice with Ed‐l2cre mice and then induced ESCC using the carcinogen 4‐nitroquinoline 1‐oxide (4‐NQO). Here, RTN4IP1 is linked to esophageal squamous cell carcinoma.