In order to provide novel insight into our understanding of the molecular mechanism of different drugs in treating colon cancer, RNA‐seq data from a p53‐wt HCT116 and p53‐mutant DLD‐1 CRC cells treated with PRIMA‐1met, L‐OHP, and PRIMA‐1met plus L‐OHP were further analyzed. The gene discussed is TP53; the disease is malignant colon neoplasm.