In the IL-8 treated group and F. nucleatum coculture group, ZEB1 expression was significantly increased compared to control, while the increase in ZEB1 expression induced by F. nucleatum was reversed after treatment with the IL-8 receptor inhibitor Reparixin (Fig. 7A, B), indicating that F. nucleatum can promote ZEB1 expression in colorectal cancer cells via the IL-8 pathway. The gene discussed is CXCL8; the disease is colorectal cancer.