Decreased mRNA and protein levels of insulin receptor β (qPCR: Insr; protein: IRβ) and increased Akt2 mRNA and total AKT protein levels in the Yoyo group, compared to Chow and/or HFD > Chow mice (Fig. 4e, f, g) are further highlighting an impaired glucose metabolism and insulin resistance after weight cycling. Here, AKT1 is linked to Insulin resistance.