Moreover, principal component analysis of single-cell RNA-sequencing data of 65,655 GSCs and 14,207 GBM cells revealed that the area where CYP3A5 was highly expressed harbored cells that also markedly expressed cancer stem cell markers, such as PROM1, SOX2, EZH2 and NOTCH1 (Fig. 2G, S3I) [26]. This evidence concerns the gene CYP3A5 and glioblastoma.