Upon enrollment, women provided data on their medical, pregnancy, and educational history, along with whole blood and serum samples for Zika, dengue, chikungunya, syphilis, toxoplasmosis, rubella, cytomegalovirus, and herpes I and II testing (Trioplex rRT-PCR, ZIKV IgM MAC-ELISA 1.0 and 2.0, Panbio Dengue IgM Capture ELISA, TORCH). The gene discussed is CD40LG; the disease is syphilis.