ZIC1 and medulloblastoma: Cycloheximide pulse-chase assays demonstrated that SHH medulloblastoma ZIC1 mutant proteins exhibit significantly higher protein stability, as compared to WT ZIC1, or G4 medulloblastoma ZIC1 mutant proteins, suggesting that the carboxy terminus IDR exerts control over the stability of the ZIC1 protein (Fig. 7g,h).