Due to the lack of accurate, robust G4 medulloblastoma cell lines, we examined the functional importance of ZIC1/ZIC4 by overexpressing blue fluorescence protein (BFP) empty vector, ZIC1, ZIC4 or ZIC1 and ZIC4 together in D425 and D283 G3 medulloblastoma cell lines. This evidence concerns the gene ZIC1 and medulloblastoma.