However, hierarchical clustering of G3 and G4 medulloblastoma by both overall transcriptome or neuronal gene expression does not segregate tumors by ZIC1/ZIC4 expression status, suggesting that the observed repression of the ZIC1/ZIC4 locus from chromatin variants is not purely secondary to a transient developmental state in the RL-SVZ (Extended Data Fig. 4e,f). This evidence concerns the gene ZIC1 and medulloblastoma.