These results were in agreement with mean Ct values for HER-2 that have been reported in positive HER-2 tumours at different concentration levels ranging from mean Ct values of RT-qPCR of 19.45, 23.20, 26.49, 29.79, 33.35, and 36.08 [24].The Results from this current study were also very closely similar to the Ct value cut-offs that have been studied for a positive expression of ER, PR and HER-2 hormonal receptors to be 22.18667, 18.2038, and 23.69193, for ER, PR and HER-2 respectively [25]. The gene discussed is ESR1; the disease is neoplasm.