The spatial distribution of these cells in the tumor showed a significantly higher percentage of FAP + (26.5% at center vs. 36.2% at periphery, Mann-U p = 0.003), CD4 + (2.7% vs. 4.9%, p < 0.001), CD8 + (2.2% vs. 4%, p < 0.001), CD20 + (0.3% vs. 1.9%, p < 0.001), and CD4 + FOXP3 +  cells (0.18% vs. 0.31%, p < 0.001) at the tumor periphery (Figs. 1, 2 and Supplementary Figure S5). Here, CD4 is linked to neoplasm.