To comprehensively understand the quantitative spatial distribution of FAP + CAFs and their association with immune cells (T and B lymphocytes and macrophages) as well as their clinicopathological significance, we conducted multiplex immunofluorescence (mIF) analysis in formalin-fixed paraffin-embedded (FFPE) samples obtained from a cohort of 88 patients with ccRCC with over 5 years of clinical follow-up. Here, FAP is linked to nonpapillary renal cell carcinoma.