Sengun et al. (2021) collected DNA samples from multiple males and conducted mutation analysis using Sanger sequencing, revealing that mutations in the FKBPL gene may lead to male infertility. Our study elucidates the mechanism by which lncFKBPL–FKBPL promotes porcine ZGA through HSP90AA1 and CDK9, expanding the understanding of FKBPL’s functions. Whether lncFKBPL is expressed in other tissues or organs of pigs and whether the mechanism of its enhancer activity in activating FKBPL also exists in other tissues or organs remain to be further explored. This evidence concerns the gene HSP90AA1 and male infertility.