Furthermore, we speculate that the upregulation of metabolic and signal transduction pathways observed in single-cell sequencing may be intrinsically linked to the activation of cell cycle regulation, Hippo signaling pathway, MAPK signaling pathway, and PI3K-Akt signaling pathway observed in bulk sequencing, all of which jointly contribute to the proliferation and survival of tumor cells. This evidence concerns the gene AKT1 and neoplasm.