IHC analysis revealed higher PlexinD1 expression in bone metastasis relative to primary tumor samples (Fig. 2F), which was congruent with PLXND1 mRNA increases in metastasis versus primary tumors from four independent PCa clinical datasets [Vanaja (Vanaja et al, 2003), Yu (Yu et al, 2004), GSE21032, and GSE35988 (Grasso et al, 2012) (Data ref: Grasso et al, 2012)] (Fig. 2G; Appendix Fig. S1B). This evidence concerns the gene PLXND1 and posterior cortical atrophy.