SMO and posterior cortical atrophy: To further investigate whether Gli1 mediates PlexinD1 function in PCa cells, we treated control and PlexinD1-overexpressing LNCaP cells with cyclopamine, a Smo inhibitor (Chen et al, 2002), or GANT61, a small-molecule inhibitor of Gli1 for abrogating Gli1-mediated transcription at the nuclear level (Lauth et al, 2007).