Characterizing 22Rv1 tumor samples by IHC, we found lower PlexinD1 protein expression in PlexinD1-knockdown relative to control tumors, indicating effective and sustainable PlexinD1 knockdown in vivo, as well as a 67% drop of Ki-67+ cells for decreased mitotic index in PlexinD1-knockdown tumors compared with controls (Fig. 4L). This evidence concerns the gene MKI67 and neoplasm.