We also found that AR signaling negatively regulates the transcription and expression of SEMA3C and SEMA3E, as evidenced by the findings that androgen depletion with CSS medium promoted, while R1881 reduced, their mRNA levels in several androgen-responsive AR+ PCa cell lines (LNCaP, LAPC4, and VCaP), which corroborated the upregulation of these Sema3 ligands in AR-repressed C4-2BENZR mCRPC cells. Here, SEMA3E is linked to posterior cortical atrophy.