This study explored the impact of lnc-CHAF1B-2 on the proliferation, migration, and invasion of gastric cancer through bioinformatics analysis and both in vivo and in vitro experiments, with a preliminary study of the molecular signalling pathways involved, to provide a new theoretical foundation and research direction for the clinical diagnosis, treatment, and prognostic evaluation of gastric cancer. This evidence concerns the gene CHAF1B and gastric cancer.