Subsequently, we assessed the sex hormone levels (PRGE, PRL, E2, LH/FSH, TESTO, and AMH) in rat serum, which revealed significant increases in the TESTO, LH/FSH, and AMH levels in the PCOS group (P < 0.05; Fig. 4C) that was consistent with human PCOS, confirming the validity of the model. Here, PRL is linked to polycystic ovary syndrome.