To specifically define the role of RAGE in BECs in establishing DR in the context of chronic liver injury we generated a tamoxifen-inducible BEC-specific conditional Rage knockout model by crossing the R26TomHnf1b-CreER mouse strain (Jörs et al, 2015) with RAGE/EGFP reporter mice described previously (Constien et al, 2001), and applied the CDE diet protocol for 3 weeks (Fig. 1A,B) leading to toxic liver injury and intrahepatic cholestasis. This evidence concerns the gene AGER and intrahepatic cholestasis.