In the prototype cases of prostate cancer described here, leukopak-derived CTC analysis using WES reproduced the mutations identified by cancer gene mutation panels in either biopsied metastatic lesions or blood-based ctDNA sampling (e.g., PTEN and TP53), and also discovered additional potentially significant mutations in genes implicated in homologous recombination (HRR), cell cycle checkpoints and chromatin remodeling. Here, TP53 is linked to Familial prostate cancer.