Using ELISA and Legendplex, we confirmed that IFNγRKO tumours contained significantly higher levels of IFNγ and IL-6, whilst other cytokines such as IFNɑ, TNFɑ, M-CSF, IL-4, and IL-10 were similar in concentration (Fig. 3E–H and Supplementary Fig. 3B–D), showing that the increase in inflammatory cytokine milieu detected by sequencing in IFNγRKO tumours was the result of an increase in specific inflammatory cytokines. The gene discussed is IL4; the disease is neoplasm.