We found that knockdown of ESCRT-0 subunit HRS or the ESCRT-I subunit TSG101 not only decreased HBV-stimulated lysosomal trafficking of NTCP-EGFP-mCherry (Fig 3D), but also largely abolished viral infection in HepG2-NTCP cells (Fig 3E and 3F). The gene discussed is TSG101; the disease is viral infectious disease.