The final aspect of this LC3 recruitment study investigated the potential bactericidal effects of autophagy by examining phagosome/autophagosome acidification levels, both with and without IFN-γ stimulation, which is known to enhance phagosome/autophagosome maturation [78] In contrast to other studies in BMDMs [16], IFN-γ pretreatment did not affect LC3 recruitment and phagosome acidity on average between 1–12 hours post-infection in THP-1 cells. This evidence concerns the gene MAP1LC3A and infection.