The letrozole-induced PCOS group exhibited PCOS symptoms such as disturbed androgens, dyslipidemia, and ovarian histopathological changes; however, after treatment with NC-100 (oral nanocurcumin solution ingestion at a dose of 100 mg/kg body weight) and NC-200 (oral nanocurcumin solution ingestion at a dose of 200 mg/kg body weight), serum samples were obtained to measure sex hormone levels. The gene discussed is PLXNA3; the disease is polycystic ovary syndrome.