Our study found that malignant cells with C9 RGS13+ Germinal center B cells, C8 IGKV1-9+ Naive B cells, C2 ITGB1+ Memory B cells, C4 CD86+ Memory B cells, and C1 NR4A1+ Memory B cells, which are five types of B cells, all of which can act as major senders and receivers of the CD99 signaling pathway, suggesting that the CD99 signaling pathway may play a prominent role in NPC growth and development. Here, IGKV1-9 is linked to nasopharyngeal carcinoma.