We specifically examined phosphorylated tau at threonine 181 (T181) and serine 396 (S396), and found significant genotype effects for both T181 (t(10) = 12.63, p < 0.0001) and S396 (t(10) = 34.97, p < 0.0001), with higher levels evident in the 3xTg-AD than NonTg (Figure 9D). This evidence concerns the gene MAPT and Alzheimer disease.