Based on the results from in vitro studies, nude mouse xenograft models (Figure 7A) with METTL16 knockdown and overexpression plasmids were constructed to validate the role of METTL16 in CC in vivo, and the effectiveness of METTL16 overexpression and knockdown was confirmed by qPCR, WB and IHC (Figure 7B and E). This evidence concerns the gene METTL16 and cholangiocarcinoma.