Additionally, on the population of osteosarcoma stem cells, EGFR overexpression significantly lessened the inhibitory effect of DUSP3 upregulation (Figure 7G-H), whereas the promoting effect of DUSP3 downregulation on osteosarcoma stem cell population was significantly inhibited upon EGFR downregulation (Figure 7I-J). This evidence concerns the gene DUSP3 and osteosarcoma.