The current study aimed to explore the anti-tumor effects of FA-modified milk-derived exosomes loaded with c-kit siRNA on gefitinib-resistant lung cancer, both in vitro and in vivo. Moreover, the current study also attempted to elucidate the molecular mechanism underlying gefitinib resistance, specifically focusing on the involvement of the c-kit signaling pathway in driving the stemness phenotype transformation. Here, KIT is linked to lung carcinoma.