In lung cancer, tsRNAs such as 5a_tRF-Cys-GCA, 3P_tRNA-Ser-GCT-6-1, 3P_tRNA-Thr-CGT-4-1, 3P_tRNA-Arg-TCT-4-1, and 5P_tRNA-Trp-CCA-3-3 were correlated with the PD-L1 immune checkpoint and PD-L1 signaling pathway-related genes 105. The gene discussed is CD274; the disease is lung cancer.