We utilized RT-qPCR to validate the expression levels of HMGA1, SCD, SCO2, LDLR, DSC1, EMP1, ETV7, FLRT2, GRM7, HOXC5, LPA, SERPINA6, and ZNF124 in the SV-HUC-1 cell line, bladder tumor cell lines (T24, J82, UC3, 5637), and normal bladder tissues. The gene discussed is EMP1; the disease is urinary bladder neoplasm.