Here, we demonstrate that non‐small cell lung cancer (NSCLC) patient‐derived CAFs upregulate the secretion of inflammatory cytokines (IL6, LIF, IL33, GM‐CSF, IL1ra) and chemokines (CCL2, CCL3, CCL4, CCL20, CXCL8, CXCL9, CXCL10, CXCL11) in response to in vitro co‐culture with anti‐CD3/anti‐CD28‐stimulated peripheral blood mononuclear cells (PBMCs) via IFNγ and TNFα. This evidence concerns the gene IL1RN and lung cancer.