Here, we demonstrate that non‐small cell lung cancer (NSCLC) patient‐derived CAFs upregulate the secretion of inflammatory cytokines (IL6, LIF, IL33, GM‐CSF, IL1ra) and chemokines (CCL2, CCL3, CCL4, CCL20, CXCL8, CXCL9, CXCL10, CXCL11) in response to in vitro co‐culture with anti‐CD3/anti‐CD28‐stimulated peripheral blood mononuclear cells (PBMCs) via IFNγ and TNFα. Here, IL1RN is linked to non-small cell lung carcinoma.