In this case-control study among a Han Chinese population composed of 592 participators, we assessed the role of ERCC3 variants including a novel germline nonsense variant c.348C > G (p.Y116X) and two missense variants rs754010782 (p.R360C) and rs371627165 (p.V471I) in breast cancer pathogenesis. This evidence concerns the gene ERCC3 and breast cancer.