Thus, Luc activity was induced 5-8 fold in melanoma cells containing full-length proximal CD47 promoter (Construct 1) as compared to low CD47 expressing HepG2 cells (Figure 5C), while CD47 promoter region containing a reduced number of NRF-1 binding elements (Constructs 2 and 3) had marginal or no effect on reporter activity in the same cells (Figures 5C–E). This evidence concerns the gene NRF1 and melanoma.