In human NB cells, we showed that oligomannosylated EGFR levels under either 2D or 3D cell culture were unaltered, and that EGFR from BE(2)-C(MGAT1−/−) were more prone to higher levels of autophosphorylation with or without EGF stimulation, which is likely why BE(2)-C(MGAT1−/−) cells were able to undergo EGF-stimulated proliferation while BE(2)-C cells were not. The gene discussed is MGAT1; the disease is neuroblastoma.