To further explore the interaction between miR-200a-5p and AMPK within the context of hepatic lipid accumulation, we established a cellular model of lipid overload using a fatty acid mixture of oleic and palmitic acids in a 2:1 ratio (PA group), simulating conditions reminiscent of high-fat diet-induced hepatic steatosis. The gene discussed is PRKAA2; the disease is Hepatic steatosis.