TNF and neoplasm: Analysis of T-cell polyfunctionality (detected by ICS for the effector molecules IFNγ, TNFα, perforin, and granzyme B) and the degranulation marker CD107a in the spleen, draining lymph node and tumor revealed signs of T-cell exhaustion in the group that received only αPD-1 therapy and in the untreated group (Supplemental Fig. 3D–F).