To monitor immune responses longitudinally from initial priming until memory formation, we again performed LS CoAT and LS/c-diGMP CoAT immunizations for Adpgkmut and another cancer neoepitope (Alg8mut) and found that c-di-GMP-encapsulated liposomes favored not only the generation of higher frequencies of primary CD8 T cells but also higher frequencies of secondary T cells after CoAT boosting (Fig. 2F). The gene discussed is CD8A; the disease is cancer.