Therefore, the aim of this study was to develop an in silico strategy for the identification of T. gondii peptides restricted to three HLA-I (HLA-A*02, HLA-A*24, and HLA-B*35) and to perform ex vivo evaluation of PBMC from individuals with chronic-asymptomatic toxoplasmosis. The gene discussed is HLA-A; the disease is toxoplasmosis.