Given that HLJ1 deficiency potentially alters hepatic gene expression patterns related to xenobiotic metabolism and chemical-induced tumor incidence, we utilized diethylnitrosamine (DEN), a well-established carcinogen that induces mouse liver cancer resembling human HCC (Lee et al. 2004), to clarify the role of HLJ1 in hepatocarcinogenesis. This evidence concerns the gene DNAJB4 and liver cancer.