Overall, ROC curve analysis confirmed the reliable diagnostic value for selected EV markers in AML patients, including CD4, CD105, and HLA-DRDPDQ, showing the best cutoff for CD44 MFI on EVs discriminating AML patients from HD subjects (AUC: 0.90, p < 0.0001; Fig. 4c). Here, CD4 is linked to acute myeloid leukemia.