We show that NT GSDMD electroporation does not lead to a decrease in tumor growth, not even when pyroptosis was enriched with IL-1β pretreatment (Fig. 8a–c and Supplementary Fig. 11b) and all animals were rapidly eliminated demonstrating that the protective effects of pyroptosis and cytokine-armed pyroptosis depend on the immune system. The gene discussed is IL1B; the disease is neoplasm.