To observe the effect of NT GSDMD-induced pyroptosis and a combination with proinflammatory cytokine on the infiltration of CD8+ cells into TME, we performed laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) on tumor tissue sections using metal-conjugated antibodies which enable spatially resolved analysis of tumor-infiltrating cells74. This evidence concerns the gene CD8A and neoplasm.