LRRK2 and Parkinson disease: The improvement of prime editing with iPE-Plus over PEmax was also observed when introducing another PD-related G2019S mutation at the LRRK2 locus35, increasing mutation rate from 9.9% to 18.5% (Fig. 4h), and a “Loxp” insertion at the HEK3 locus, increasing from 42.8% to 56.7% (Fig. 4i), without sacrificing editing purity (Fig. 4j).