Using immunohistochemistry (IHC), we evaluated the protein expression of iNOS and phospho-Akt in tissues from breast cancer PDX models and found that MpBC PDXs predominantly had more co-expression of high/moderate staining of iNOS and phospho-Akt relative to other breast cancer PDX subtypes (Fig. 2E). The gene discussed is AKT1; the disease is breast carcinoma.