The results showed that, compared to the groups without Era, blocking the GPX4 pathway led to reduced GSH (Figures 4B, 5B), increased MDA (Figures 4C, 5D), elevated Fe2+ (Figures 4D, 5E), increased mitochondrial membrane potential (Figure 4F), decreased cell viability and elevated NT-proBNP (Figures 4E, 5J), enlarged heart (Figure 5G), increased heart mass (Figure 5F), reduced LVEF (Figures 5H, I), and increased myocardial fibrosis (Figures 5K, L), with aggravated mitochondrial shrinking and cristae fusion (Figure 5C). Here, GPX4 is linked to Myocardial fibrosis.