Compared to non-irradiated tumor cells, TREX1 and γ-H2AX expression levels were elevated in tumor cells subjected to partial or whole irradiation at 12 Gy, indicating that while TREX1 expression in 4T1 tumor cells irradiated at 12 Gy can reduce cytoplasmic dsDNA accumulation, it does not fully degrade the dsDNA or prevent STING activation. Here, H2AX is linked to neoplasm.