In contrast, LukS-PV treatment led to a marked decrease in HCC cell proliferation (Figs. 2E, S2F), and Wnt3a significantly attenuated the inhibitory effects of LukS-PV on HCC cell proliferation compared with LukS-PV treatment group (Figs. 2E, S2F). This evidence concerns the gene WNT3A and hepatocellular carcinoma.